Evidence of LAT1 expression in canine caput epididymis
نویسندگان
چکیده
L-type amino acid transporter 1 (LAT1), the first isotype of amino acid transport system L, transports aromatic and branched amino acids pivotal for fundamental cellular activities such cellular growth and proliferation. LAT1 expression was high only in the brain in contrast to its limited distribution and low level of expression in normal tissues. We found potent LAT1 expression in canine caput epididymis by quantitative RT-PCR and Western blotting analysis. Immnuno-histochemical examination revealed observable LAT1 in microvillous epithelial cells.
منابع مشابه
Morphological and acrosomal changes of canine spermatozoa during epididymal transit
BACKGROUND During epididymal transit, functional and structural modifications leading to full maturation enable male gametes to reach, recognize and fertilize the oocytes. In dogs, little is known on the modifications of spermatozoa during the passage in the epididymis. The aim of this study was to describe the motility, morphology and acrosomal patterns of canine spermatozoa retrieved from the...
متن کاملCurcumin Ameliorates Aflatoxin-Induced Changes in Caput and Cauda Epididymis of Mice
Background The main aim of the present study was to evaluate the ameliorative effect of curcumin on aflatoxin-induced changes in caput and cauda epididymis of mice. MaterialsAndMethods Aflatoxin was obtained by growing Aspergillus parasiticus in sucrose - magnesium sulphate - potassium nitrate - yeast extract (SMKY) liquid medium. Pure curcumin (97% purity) was purchased from Hi-Media Laborator...
متن کاملDemonstration of focal physiologic in-vivo somatostatin receptor expression in the caput epididymis of the testes on 68Ga-DOTANOC PET/CT and 177Lu-DOTATATE post-therapy whole body scintigraphy
We present the case of a 60-year-old man with metastatic neuroendocrine tumor of the ileum following ileal resection, being evaluated for 177Lu-based peptide receptor radionuclide therapy. 68Ga-DOTANOC PET/CT showed focal increased tracer uptake in the scrotal region without any morphologic changes on the corresponding CT images. Similar increased tracer uptake was seen on...
متن کاملDifferent expression of B7-H3 in the caput, corpus, and cauda of the epididymis in mouse
BACKGROUND B7-H3, a member of the B7 family of the Ig superfamily of proteins, has been detected in the epididymis, which is a storage organ related to sperm maturation. However, the characteristics of its expression in different regions of the epididymis remain unknown. Our aim was to investigate the expression of B7-H3 in the caput, corpus, and cauda of the epididymis. METHODS We extracted ...
متن کاملAssessment of epididymal sperm obtained from dromedary camel
Testicles were isolated from dromedary camels in a local slaughterhouse at breeding and non-breeding seasons. Sperms were recovered from different parts of the epididymis (caput, corpus and cauda) and stained separately on slide glasses by eosin nigrosin staining method and dried by a hair dryer and carried to the laboratory. In the lab, slides were observed for evaluation of the proportion of ...
متن کامل